We report a voltammetric ethanol sensor based on alcohol dehydrogenase immobilized at cerium hexacyanoferrate (II) (CeHCF(II)) nanoparticles incorporated poly-L-lysine (PLL) film modified glassy carbon electrode (GCE). The electrostatic interactions between PLL, CeHCF(II) nanoparticles and ADH layers provide good stability to the nanocomposite film. The as-prepared CeHCF(II) nanoparticles and ADH/PLL/CeHCF/PLL films were characterized by field emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM) study. FESEM results confirmed that CeHCF(II) nanoparticles are 25–30 nm in size. Powder X-ray diffraction (XRD) results demonstrated that CeHCF(II) nanoparticles are crystalline and belongs to hexagonal symmetry with a space group of P63/m. The fabricated ADH/PLL/CeHCF/PLL nanocomposite film modified GCE exhibits excellent electrocatalytic response towards ethanol in 0.1 M KNO3 solution (pH 7). The linear concentration range and sensitivity values are 0.04 to 0.23 mM and 13.86 μA mM-1 cm–2, respectively. The proposed ADH/PLL/CeHCF/PLL film exhibits good stability, high selectivity and it detects ethanol present in commercially available red wine samples with good recovery.