An enzyme-free hydrogen peroxide (H2O2) biosensor based on MWCNT–PEDOT film modified electrode
has been successfully performed on glassy carbon electrode (GCE) and indium tin oxide (ITO) electrode.
At an applied potential of −0.5V vs. Ag/AgCl, the MWCNT–PEDOT electrode exhibited linearly dependence
on H2O2 concentration in the range of 0.1–9.8mM. It can be observed two significantly linear
sections. One shows sensitivity of 943MmM−1 cm−2 with signal/noise of 6; and the other one shows
sensitivity of 174MmM−1 cm−2 with signal/noise of 4 in pH 7 PBS. It also presented excellent stability
at room temperature, with a variation of response current less than 5% over 30 days. Moreover, the sensor
was characterized by cyclic voltammetry (CV), scanning electronic microscopy (SEM), atomic force
microscopy (AFM), and different pulse voltammetry (DPV). This sensor also can simultaneously detect
AA, DA, and UA and can be utilized to develop multifunctional biosensors.