With the aim of improving blood compatibility of low density polyethylene (LDPE) films, an effective low-pressure plasma technology was employed to functionalize the LDPE film surfaces through in-situ grafting of acrylic acid (AAc). Subsequently, the molecules of poly(ethylene glycol) (PEG) and chitosan (CHI) were immobilized on the surface of grafted LDPE films. The unmodified and modified LDPE films were analyzed using various characterization techniques such as contact angle, atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and X-ray photo electron spectroscopy (XPS) to understand the changes in surface properties such as hydrophilicity, surface topography and chemical composition, respectively. Furthermore, LDPE films have been subjected to an ageing process to determine the durability of the plasma assisted surface modification. The blood compatibility of the surface modified LDPE films was confirmed by in vitro tests. It was found that surface modified LDPE films show better hydrophilic behavior compared with the unmodified one. FTIR and XPS results confirm the successful immobilization of CHI on the surface of LDPE films. LDPE films showed marked morphological changes after grafting of AAc, PEG and CHI which were confirmed through AFM imaging. The in vitro blood compatibility tests have clearly demonstrated that CHI immobilized LDPE films exhibit remarkable anti thrombogenic nature compared with other modified films. Surface modified LDPE films through low-pressure plasma technique could be adequate for biomedical implants such as artificial skin substrates, urethral catheters or cardiac stents, among others.