Multi-walled carbon nanotubes (MWCNTs) with glucose oxidase (GOx) are directly fabricated on glassy carbon electrode (GCE) and indium tin oxide electrode (ITO) by simple process. The MWCNTs/GOx modified ITO electrode surface has been studied in detail using scanning electron microscopy (SEM) and atomic force microscopy (AFM). This MWCNTs/GOx film modified GCE effectively exhibits the electro oxidation signals for the detection of glucose. MWCNTs provided large surface area for GOx base on modified electrode. The cyclic voltammetry (CVs) has been used for the measurement of electroanalytical properties of analytes by means of modified electrodes. The power densities of glucose biofuel cell based on the MWCNTs/GOx electrode was 65 W/cm2 at 0.58 V, respectively. The biofuel cell showed highly stable output in long term performance. Efforts are underway to improve the interface transfer to achieve higher potential and current output