The coating of hesperidin, a traditional Chinese medicine ingredient, was immobilized to the surface of stainless steel (SS) which was often used in the cardiovascular implant materials and evaluated for its blood compatibility in vitro. The immobilized coating showed prolonged activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) values compared with stainless steel control. The platelet adhesion and activation test on the hesperidin coating also showed significantly less data compared with the control. The test on conformational change of fibrinogen (fgn) demonstrated the hesperidin coating could reduce this parameter compared with the bare SS. It can be summarized that the hesperidin coating can effectively improve the blood compatibility of the SS surface. We envisage that this coating will provide a potential and effective selection for blood contact devices.