Novel platinum and silver decorated multi-walled carbon nanotubes (PtAg/MWCNTs) have been successfully prepared on electrode surface for nonenzymatic glucose detection. X-ray diffraction (XRD) and atomic force microscopy (AFM) analyses reveal that the Pt and Ag NPs were successfully deposited on the MWCNTs in this hybrid composite. In neutral condition, the electrode shows good activity towards glucose oxidation with low overpotential (-0.35 V vs. Ag/AgCl) and a current response that is 2.5–20 times greater than that obtained using Pt/MWCNTs/GCE and Ag/MWCNT/GCE. The optimised condition based on current response is found at pH 1. Voltammograms indicate a linear range of 1–25 mM with sensitivity of 115.8 μA mM-1 cm-2. This electrode can effectively analyse glucose concentration in bovine serum albumin samples. It shows advantages with low overpotential, high sensitivity, good stability, and low cost.