Conductive composite films with the combination of nanomaterials such as multi-walled carbon nanotubes (MWCNTs), nano platinum (Pt), nano gold (Au), nafion (NF) and hydroxypropyl-β-cyclodextrin (HPβCD) as catalysts have been synthesized on glassy carbon, gold and indium tin oxide electrodes (ITO) by potentiostatic methods. Both, the cyclic
voltammetry and differential pulse voltammetry have been used for the measurement of electroanalytical properties of neurotransmitters and nucleotide bases by means of nanocomposite film modified electrodes. The presence of MWCNTs in the composite films enhances the active surface coverage concentration of catalysts. The presence of
nanoparticles in the films enhances the functional properties and produces an overall increase in the sensitivity of the modified electrodes. One such composite film (MWCNTs-NF-PtAu) exhibit promising catalytic activity towards the oxidation of mixture of biochemical compounds such as ascorbate anion, epinephrine and urate anion in aqueous buffer solution (pH 6.75). Well separated voltammetric peaks have been obtained for ascorbate, epinephrine and urate anions with the peak separations of 0.222 and 0.131 V. Another type of composite film (MWCNTs-Au-HPβCD) exhibits promising electrocatalytic activity towards the oxidation of tyrosine (TYR), guanine (GU), adenine (AD) and thymine (THY) present in pH 7.4 aqueous solutions. Well separated voltammetric peaks have been obtained between TYR and GU (80 mV), GU and AD (290 mV), AD and THY (185 mV). Electrochemical quartz crystal microbalance, scanning electrochemical microscope, atomic force microscope and scanning electron microscope techniques have been used for the electrochemical characterizations and surface morphology studies