We have first reported the modification of ractopamine electrodeposition films. In this paper, the electrochemical oxidation of ractopamine in different pHs and conditions at the bare glassy carbon electrode. The bare glassy carbon electrode also exhibits a promising enhanced electroanalytic activity towards the oxidation of ractopamine. Different methods were used for the formation of poly-ractopamine films and the deposition on electrode. Cyclic voltammograms (CVs), Linear sweep voltammetry (LSV) and Electrochemical impedance spectroscopy (EIS) are used for the determination of ractopamine and the apparent diffusion coefficient values for these compounds at different concentration as it gives some information about the kinetics of charge transfer during the redox reactions of these compounds. Finally, we have studied the surface morphology of the modified electrode using atomic force microscopy (AFM), which revealed that ractopamine is coated on ITO.