Platinum nanoparticles (PtNPs) are directly fabricated on glassy carbon electrode (GCE) and indium tin oxide electrode (ITO) by simple electrochemical deposition process. The Pt nanoparticles modified ITO electrode surface has been studied in detail using scanning electron microscopy (SEM) and atomic force microscopy (AFM). This PtNPs film modified GCE effectively exhibits the electro oxidation signals for the detection of H2O2. Poly Tyramine (pTy) provided –NH3 group for Choline oxidase (ChOx) base on modified electrode. The Cyclic voltammetry (CVs), Linear Sweep voltammetry (LSV) and Differential pulse voltammetry (DPVs) has been used for the measurement of electroanalytical properties of analytes by means of modified electrodes. Especially, the proposed PtNPs/pTy/ChOx film modified GCE successfully showed oxidation for the detection of choline. This sensor shows excellent performance with a sensitivity of 69717 μA mM-1 cm-2, a linear range of 2.1×10-7 – 1.9×10-4 M, and a detection limit of 1.8×10-7 M. The proposed PtNPs/pTy/ChOx film modified electrode also retains the advantage of easy fabrication, high sensitivity and good repeatability. Future, this type of PtNPs/pTy/ChOx film modified electrode supports the selective detection of choline in clinical diagnosis.