Uniform reduced graphene oxide-wrapped WO₃ nanowire nanocomposite (RGO–WO₃ nanowire) was prepared by a one-step solvothermal method. The resulting RGO–WO₃ nanowire was first characterized by using FT-IR, XRD, AFM and SEM, and then was used to modify the glassy carbon electrode (GCE). The electrochemical behavior of honokiol on the unmodified GCE, WO₃ nanowire modified GCE and RGO–WO₃ nanowire modified GCE was compared. The RGO–WO₃ nanowire composite has remarkably enhanced the electrochemical character of honokiol, with a significantly improved current value of oxidation peak in the composite modified GCE. After 2-min under open circuit, a linear range from 1.0×10⁻⁸ to 2.0×10⁻⁵ mol/L and detection limit of honokiol as low as 8×10⁻⁹ mol/L were obtained. This analysis method has a high recovery ratio of honokiol up to 103%.