Poly taurine nano gold film has been fabricated on glassy carbon electrode (GCE) and ITO using cyclic voltammetry. Fabricated poly taurine nano gold film modified GCE has been successfully employed for the detection of arsenic in lab analysis and real drinking water sources like mineral water, spring water and tap water samples etc. The poly taurine nano gold film modified GCE successfully detects the arsenic in the linear range of 6 to 28 μM with the detection limit of 0.46 μM in lab samples. Also, the proposed film successfully detects the arsenic in drinking water sources like spring water samples (1 and 2) in the linear range of 10 to 34 μM with a detection limit of 0.91 μM, 5to 28 μM with a detection limit of 0.58 μM, respectively.