The aim of this work was to study the photoelectrochemical optimal conditions for red cabbage extract as natural dye to develop a dye-sensitized solar cells (DSSC). Red cabbage extract were characterized by various methods, such as electrochemical impedance spectroscopy (EIS), UV–Vis and cyclic voltammetry (CVs). DSSC is fabricated from a combination of relatively popular materials containing TiO2 photoelectrode, natural dye, electrolyte containing I61/I361 redox mediator, and counter electrode. The use of suitable pH for red cabbage improves the DSSC performance. The results showed that the increase of efficiency due to the purified and immersed time of natural dye content greatly increased the specific activity and total load volume.